|

Part
1 - Introduction
Part
2 -
Scope of Work
Part
3 - Tasks
Part
4 -
1st Quarterly Report
|
|
|
|
|
San Juan Creek - Reports and Studies
|
 |
San Juan Creek Watershed Bacterial Study
Orange County Public Health Laboratory
Contract Scope of Work - Tasks
Part 3
|
| |
 |
| |
Task 1. Project Management and Administration
1.1 Provide all technical and administrative services as needed
for contract completion; monitor, supervise and review all work performed; and coordinate
budgeting and scheduling to assure that the contract is completed within budget,
on schedule and in accordance with approved procedures, applicable laws and regulations.
1.2 Ensure that contract requirements are met through completion
of Quarterly Progress Reports submitted to the Contract Manager by the 10th of the
month following the end of the calendar quarter (March, June, September, and December)
and through regular communication with the Contract Manager. The Quarterly Progress
Reports shall describe activities undertaken and accomplishments of each task during
the quarter, milestones achieved, and any problems encountered in the performance
of the work under this contract. The description of activities and accomplishments
of each task during the quarter shall be sufficient in detail to provide a basis
for the payment of invoices and shall be translated into percent of task completed
for the purpose of calculating invoice amounts.
1.3 Document steps taken in soliciting and awarding the subcontract
and submit them to the Contract Manager for review.
1.4 Project Survey Form - At the completion of the project
and prior to final payment, the Project Director shall complete and provide a project
survey form to be included with the final report.
Task Products:
Quarterly progress reports, subcontractor selection, project
survey form.
Task 2. Assemble a Technical Advisory Committee
Form a Technical Advisory Committee (TAC) comprised of technical
experts representing various agencies (Orange County Health Care Agency, Regional
Water Quality Control Board and California Department of Health Services), and the
scientific community. The Contractor will facilitate the meetings. The TAC will be
request to provide recommendations for development of the storm drain and tributary
sampling plan (Sampling Plan).
2.1 Meeting Agendas - The Contractor will develop and distribute
written meeting agendas. Discussion items will include the Sampling Plan.
2.2 Meeting Participants - At a minimum, the following representatives
shall be invited to participate in the meetings:
Agency Technical Representatives of
Orange County Health Care Agency;
Regional Water Quality Control Board, San Diego Region;
California Department of Health Services;
2.3 Meetings - The TAC group will meet on an as-needed basis
throughout this project. Contact may also be accomplished by email and telephone.
At the meetings, the details of the Sampling Plan will be finalized (e.g., sampling
sites, sampling periods, water quality constituents and so on).
Task Products:
2.1 Meeting Agenda(s) and minutes
2.3 TAC participant sign-up sheet(s)
Task 3. Bacteriological Survey of Watershed and Adjacent Beach
Recreational Water (Phase 1)
3.1 Sampling Plan for Storm Drains and Tributaries - Prepare
a Sampling Plan for dry weather site locations. The Sampling Plan shall identify
who will collect the samples and the time and frequency of sample collection at
each location specified. The Sampling Plan shall provide for the analysis of the
samples for total and fecal coliform and enterococcus bacteria. Bacteriological
testing will include total coliform and fecal coliform by MTF (Multiple Tube Fermentation)
or membrane filtration and enterococcus by Enterolert or membrane filtration. A
Quality Assurance/Quality Control (QA/QC) program shall be developed and implemented
as part of the Sampling Plan.
3.2 Conduct Phase 1 Storm Drain and Tributary Sampling - The
Sampling Plan developed in Task 3.1 will be conducted. This task includes sample
collection and analyses of bacteriological samples, plus any other data relating
to water quality and conditions at the time of sampling. Sampling data may include
data on flow rate, electrical conductivity (EC), total suspended solids (TSS), or
other parameters as prescribed within the Sampling Plan. The County of Orange Public
Health Laboratory shall conduct the sampling, testing, and analysis.
3.3 Watershed Bacteriological Survey Report - Prepare a report
that describes the results from Task 3.2. The report shall include analytical results,
as well as other data on water quantity and quality. The report shall also identify
problem areas to be further studied in a detailed bacteriological survey. The report
shall be submitted within a timely manner of receiving the analytical results.
Task Products:
3.1 Watershed Sampling Plan
3.2 Conduct Watershed Storm Drain and Tributary Sampling Plan
3.3 Watershed Bacteriological Survey report
|
|
|
Task 4. Detailed Bacteriological Survey of Identified Problem Areas (Phase 2)
4.1 Sampling Plan for Problem Areas - Prepare a Sampling Plan
for problem areas. The Sampling Plan shall identify who will collect the samples
and the time and frequency of sample collection at each location specified. The
sampling Plan shall identify collection sites from at least one known problem area
and a second area geographically removed. The Sampling Plan shall be determined
by Public Health Laboratory staff and with input from PFRD, Environmental Health
and the Technical Advisory Committee. The Sampling Plan shall provide for the analysis
of the samples for total and fecal coliform, E. coli, and enterococcus bacteria.
The Sampling Plan shall provide for the collection of approximately 200 samples
over approximately 10 weeks and for the saving isolates of E. coli and enterococcus
for analysis in Phase 4 of this study.
4.2. Conduct Phase 2 Bacteriological Survey - The Sampling
Plan developed in Task 4.1 will be conducted. This task includes sample collection
and generation of E. coli and enterococcus isolates from bacteriological samples,
plus any other data relating to water quality and conditions at the time of sampling.
Sampling data may include data on flow rate, electrical conductivity (EC), total
suspended solids (TSS), or other parameters as prescribed within the Sampling Plan.
The County of Orange Public Health Laboratory shall conduct the sampling, testing,
and analysis.
4.3. Problem Area Bacteriological Survey Report - Prepare
a report that describes the results from Task 4.2. The report shall include analytical
results, as well as other data on water quantity and quality. The report shall also
identify the temporal and geographic boundaries of the problem at the problem areas.
The report shall be submitted within a timely manner of receiving the analytical
results.
Task Products:
4.1 Problem Area Sampling Plan
4.2 Conduct Problem Area Sampling Plan
4.3 Problem Area Bacteriological survey report
Task 5. Source Identification by ARA and Ribotyping: Library Preparation
and Technique Accuracy Determination. (Phase 3)
5.1 Construct Bacterial Isolates Library from Known Species.
- Construct a local bacteria isolates library. The library will contain strains
of E. coli and enterococcus bacteria isolated from approximately 100 samples of
known species, including humans and animal species that may be a major contributor
to bacteria pollution in the watershed. For the ARA (Antimicrobial Resistance Analysis)
technique approximately 500 strains of each bacterium will be developed by picking
5 isolates from each sample for each bacterium. For the Ribotyping technique approximately
100 strains of E. coli per species will be developed from 1 isolate per sample.
Isolates shall be frozen to permit analysis by other techniques in the future. Bacteria
will be isolated, identified, and frozen by the public health laboratory staff.
A Quality Assurance (QA) program shall be developed and implemented as part of the
library preparation that includes reproducibility and accuracy tests.
5.2 Accuracy Determination Report of Bacterial Source Identification
Methods - Prepare a report on the accuracy of the source identification methods
based on the results obtained from the accuracy tests. Final accuracy of each source
identification technique shall be determined for each species in the local bacteria
isolate library. The same strains will be used for both typing techniques.
Task Products:
5.1 Bacteria Isolates Library
5.2 Report of Source Identification Accuracy Determination
Task 6. Source Identification by ARA and Ribotyping: Source Identification of Watershed
Isolates. (Phase 4)
6.1 Source Identification Report - Prepare a source identification
report that describes, identifies and quantifies the major sources of bacteriological
contamination in the problem areas of the watershed based on the results obtained
from the bacterial source tracking identified above. The report shall include an
explanation of the work performed and results of all phases of the study. The report
shall include the analytical results for the source identification methods as well
as other data on water quantity and quality. The report shall include the Quality
Assurance/Quality Control (QA/QC) tests performed. The report shall include a discussion
of the data that has been obtained and the implementation measures which may be
used to correct the identified loadings. The report shall identify and compare results
of both the ARA and Ribotyping methods and include a discussion of the comparison
of the two bacterial source tracking techniques. A final draft report shall be submitted
upon completion of all phases of the study and shall not be delayed pending final
preparation of the manuscript for the peer-reviewed journal.
6.1.b Interim Source Identification Report - Prepare an interim
source identification report by March 1, 2002 that describes the status of the source
identification of watershed isolates. The interim report shall include results of
source identification performed to date and the status and expected conclusion date
of Phase 4 of the study.
Task Products:
6.1 Source Identification Report
6.1.b Interim Source Identification Report
|
|
|
|
C. Schedule of Completion Dates.
| Task # |
Task Description
|
Completion Date |
| Task 1 |
Project Management & Administration |
On-going
|
| Task 2 |
Assemble Technical Advisory Committee |
4/01
|
| Task 3 |
Phase 1 - Bacteriological Survey of Watershed
and Adjacent Beach Recreational Water |
9/01
|
| Task 4 |
Phase 2 - Detailed Bacteriological Survey of
Identified Problem Areas |
11/01
|
| Task 5 |
Phase 3 - Source Identification by ARA and
Ribotyping: Library Preparation and Technique Accuracy Determination |
2/02
|
| Task 6 |
Phase 4 - Source Identification by ARA and
Ribotyping: Source Identification of Watershed Isolates |
5/02
|
D. Reports
1. Not later than June 1, 2001 and quarterly thereafter, during
the life of this agreement, the Project Director shall provide a written progress
report to the Contract Manager describing activities undertaken, accomplishment
of milestones, and any problems encountered in the performance of the work under
this agreement, and delivery of intermediate products, if any.
2. The Project Director shall submit to the Contract Manager
reports upon the completion of each Phase of the Study and an interim report for
Phase 6 as described in this Scope of Work. In the event completion dates for the
Phases are delayed, then a progress report containing the results of the work performed
in the 3 months following the last report shall be submitted.
3. Not later than February 28, 2002, the Project Director
shall submit to the Contract Manager two (2) copies of a draft final report describing
the work performed pursuant to Section B of this Exhibit for review and comment.
4. Within four (4) weeks of receipt of the draft report, the
Contract Manager will submit his final comments to the Project Director.
5. On or before the termination date of this agreement, the
Project Director shall submit to the Contract Manager for approval one (1) reproducible
master and two (2) copies of the final report containing the results of the work
performed and addressing the comments submitted to the Project Director by the Contract
Manager.
6. The report will not be considered final until approved
and accepted by the Contract Manager.
A. BUDGET:
|
Task
|
Item
|
Cost
|
|
1
|
Project Management and Administration |
$0.00
|
|
2
|
Assemble Technical Advisory Committee |
$0.00
|
|
3
|
Phase 1 - Bacteriological Survey of Watershed
and Adjacent Beach Recreational Water |
$27,000.00
|
|
4
|
Phase 2 - Detailed Bacteriological Survey of
Identified Problem Areas |
$42,300.00
|
|
5
|
Phase 3 - Source Identification by ARA and
Ribotyping: Library Preparation and Technique Accuracy Determination |
$57,600.00
|
|
6
|
Phase 4 - Source Identification by ARA and
Ribotyping: Source Identification of Watershed Isolates |
$61,600.00
|
|
|
Total Cost
|
$188,500.00
|
B. INVOICING PROCEDURES:
The Contractor shall be paid quarterly in arrears, upon
submission of an original and two copies of the invoice which properly details all
charges, expenses, direct and indirect costs. Invoices shall be submitted to:
Chris Means
California Regional Water Quality
Control Board, San Diego Region
9771 Clairemont Mesa Blvd., Suite A
San Diego, CA 92124-1324
1. The original and one (1) approved copy of the invoice
or payment request will be forwarded to the State Water Board's Accounting Operations
Section by the Contract Manager. Contractors who are certified as small businesses
or recognized as non-profit organizations by the Office of Small Business Certification
and Resources will be paid in accordance with California Government Code, Title
1, Section 926.15. Invoices for all other contractors shall be paid within 45 calendar
days. In either situation, payment of any invoice will be made only after receipt
of a complete, adequately supported, properly documented and accurately addressed
invoice or payment request. Failure to use the address exactly as provided above
may result in return of the invoice or payment request to the Contractor. Payment
shall be deemed complete upon deposit of the payment, properly addressed, postage
prepaid, in the United States mail. All invoices must be approved by the Contract
Manager.
2. Payments prior to satisfactory completion of all work required
by the agreement shall not exceed, in the aggregate, ninety percent (90%) of the
total earned with the balance to be paid upon satisfactory completion of the agreement,
and provided, further, that the State Water Board shall retain from the Contractor's
earnings for each period for which payment is made an amount equal to ten percent
(10%) of such earnings, pending satisfactory completion of the agreement.
3. The invoice shall contain the following information:
(a) The word "INVOICE" should appear in
a prominent location at the top of the page(s);
(b) Printed name of the Contractor;
(c) Business address of the Contractor, including P.O.
Box, City, State, and Zip Code;
(d) Name of State Water Board/Regional Water Board being
billed;
(e) The date of the invoice;
(f) The number of the agreement upon which the claim is
based; and
(g) An itemized account of the services for which the State
Water Board is being billed:
(1) The time period covered by the invoice, i.e.,
the term "from" and "to";
(2) A brief description of the services performed;
(3) The method of computing the amount due. The invoice shall detail the percentage
of each task completed during the invoice period multiplied by the agreed upon
cost to perform each task, as specified in the Budget less the ten percent (10%)
withhold, if any;
(4) The total amount due; this should be in a prominent
location in the lower right-hand portion of the last page and clearly distinguished
from other figures or computations appearing on the invoice; the total amount
due shall include all costs incurred by the Contractor under the terms of this
agreement; and
(5) Original signature of Contractor (not required
of established firms or entities using preprinted letterhead invoices).
 
|
|
|
|